z-Ch. 5 & 6 Protein and DNA

WD40
Function: protein-protein interactions, a stable propeller-like platform to which proteins bind either stably or reversibly
EF-hand
Function: Calcium binding Specificity: Ca2+
SNARE
Function: protein-protein interactions in intracellular membrane fusion Specificity: other SNARE domains
SH3
Function: protein-protein interactions Specificity: proline-rich sequences
SH2
Function: protein-protein interaction
Chromo
Function: protein-protein interactions in chormatin remodeling Specificity: methylated lysine
Bromo
Function: protein-protein interactions in chromatin remodeling Specificity: acetylated lysine
Acetylation
Loosening up activation
SUMOylation
Transport
Ubiquitination
Degridation
Methylation
Silancing
Phosphorylation
Activation
1nm
= 10A
Adenine
[image]
Guanine
[image]
Cytosine
[image]
Thymine
[image]
Major groove Minor groove
22A 12A
Tm
Half DNA in single strand form
Higher with higher GC content and higher salt content
Topoisomerase
ss/ds breaks changes supercoiling
Type I: ss
Type II: ds requires ATP to reset
Gyrase
type of topoisomerase introduces negative supercoils or relaxes positive supercoils
Helicases
separate strands of DNA during synthesis, transcription, recombination, repair
kDa -> AA
X 9
AA -> kDa
/9
Phosphofructokinase
Fructose 6-phosphate to fructose-1,6-bisphosphate
fructose-1,6-bisphosphate
Glyceraldehyde 3-phosphate and dihydroxyacetone phosphate
Pyruvate kinase
phosphoenolpyruvate to pyruvate

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